CMV IgM ELISA Kit

Intended use

The CMV IgM assay is an enzyme-linked immunosorbent assay (ELISA) for the in vitro quantitative determination of IgG antibodies to cytomegalovirus (CMV) in Human serum and plasma.

Summary

Cytomegalovirus (CMV) (from the Greek cyto-, "cell", and megalo-, "large") is a genus of viruses in the order Herpesvirales, in the family Herpesviridae, in the subfamily Betaherpesvirinae. Humans and monkeys serve as natural hosts. There are currently eight species in this genus including the type species, human cytomegalovirus (HCMV, human herpesvirus 5, HHV-5), which is the species that infects humans. Diseases associated with HHV-5 include mononucleosis, and pneumonia.In the medical literature, most mentions of CMV without further specification refer implicitly to human CMV. Human CMV is the most studied of all cytomegaloviruses.

Test principle

Capture method. Total duration of assay: 70 minutes

This kit uses capture ELISA principle to detect CMV IgM. Polystyrene microwell strips are pre-coated with mouse anti-human IgM (anti-µ chain specific). During the first incubation step, all IgM antibodies binds to mouse anti-human IgM monoclonal antibody coated in microplate including the Anti-CMV IgM, the wells are washed to remove unbound serum proteins, and CMV antigens conjugated to the enzyme horseradish peroxidase (HRP-Conjugate) are added. During the second incubation step, if there is CMV IgM antibody in sample, HRP- conjugated antigens will be bound to the anti-human CMV IgM complexes previously formed and the unbound HRP-conjugate is then removed by washing. Chromogen solutions containing Tetramethylbenzidine (TMB) and urea peroxide are added to the wells and in presence of the anti-human IgM -antibody(IgM- antigen (HRP) immunocomplex, the colorless Chromogens are hydrolyzed by the bound HRP conjugate to a blue-colored product. The blue color turns yellow after stopping the reaction with sulfuric acid. The amount of color intensity can be measured and it is proportional to the amount of antibody captured in the wells, and to the amount of antibody in the sample respectively. .

Technical Parameters:

Specimen collection and preparation

• Human serum or plasma is recommended for this assay.

• Cap and store the samples at 18-25 ℃ for no more than 8 hours. Stable for 7 days at 2-8℃, and 1 month at -20℃. Freeze only once.

• Do not use heat-inactivated samples.

• Sediments and suspended solids in samples may interfere with the test result which should be removed by centrifugation. Ensure that complete clot formation in serum samples has taken place prior to centrifugation.

• Avoid grossly hemolytic, lipemic or turbid samples.

FAQ:

Q: What is the brand name of this test kit?

A: The brand name of this test kit is Biovantion.

Q: Where is this test kit manufactured?

A: This test kit is manufactured in China.

Q: Is this test kit certified?

A: Yes, this test kit is certified with ISO13485.

Q: What is the minimum order quantity for this product?

A: The minimum order quantity for this product is 10.

Q: What is the packaging details for this product?

A: The packaging details for this product is CARTON.

Q: What is the delivery time for this product?

A: The delivery time for this product is 7-15 days.

Q: What are the payment terms for this product?

A: The payment terms for this product is TT 100% PAYMENT.

Q: What is the supply ability for this product?

A: The supply ability for this product is 100000.

Q: Is the price of this product negotiable?

A: Yes, the price of this product is negotiable.