| Category |
Details |
| Product Description |
FSH Overview |
| |
FSH (follicle stimulating hormone), together with LH (luteinizing hormone), belongs to the gonadotropin family. |
| |
FSH and LH regulate and stimulate the growth and function of the gonads (ovaries and testes) synergistically. |
| |
FSH is a glycoprotein with a molecular weight of approx. 32,000 daltons, consisting of α- and β-chains. |
| |
Function in Women: Controls the menstrual cycle by stimulating follicle growth and maturation, and estr0gen production. |
| |
Function in Men: Induces spermatogonium development. |
| |
Clinical Relevance: High FSH levels during menopause, and abnormal levels can indicate hypothalamus-pituitary-gonads dysfunction. |
| |
Used in Diagnosis: Menopausal syndrome, polycystic ovaries (PCO), amenorrhea, and azoospermia. |
| Test Principle |
Method |
| |
Based on the sandwich immunoassay principle. |
| |
Procedure: |
| |
1. Sample, anti-FSH coated microwells, and enzyme-labeled anti-FSH are combined. |
| |
2. Incubation allows FSH in the sample to form a complex with the antibodies. |
| |
3. After washing, a solid-phase complex is formed. |
| |
4. Substrate is added, and a chromogenic reaction occurs, proportional to FSH concentration. |
| |
Assay Duration: 80 minutes |
Technical Parameters:
| Product Name |
FSH Elisa Test |
| Manufacturer |
Biovantion |
| Test Type |
ELISA |
| Format |
Kit |
| Specimen |
Serum 50μl |
| Sensitivity |
High |
| Specificity |
High |
| Applications |
Hormones Diagnosis |
| Assay Time |
1 Hour |
| Shelf Life |
18 Months |
Materials provided:
• Coated Microplate, 8 x 12 strips, 96 wells, pre-coated with mouse monoclonal Anti-FSH.
• Calibrators, 6 vials, 1 ml each, ready to use; Concentrations: 0(A), 5(B), 20(C), 50(D), 100(E) and 200(F) mIU/mL.
• Enzyme Conjugate, 1 vial, 11 mL of HRP(horseradish peroxidase) labeled mouse monoclonal Anti-FSH in Tris-NaCl buffer containing BSA (bovine serum albumin). Contains 0.1% ProClin300 preservative.
• Substrate, 1 vial, 11ml, ready to use, (tetramethylbenzidine) TMB.
• Stop Solution, 1 vial, 6.0 ml of 1 mol/l sulfuric acid.
• Wash Solution Concentrate, 1 vial, 25 ml (40X concentrated), PBS-Tween wash solution.
• IFU, 1 copy.
• Plate Lid: 1 piece.
Storage:
• Store at 2-8℃.
• Place unused wells in the zip-lock aluminum foiled pouch and return to 2-8 °C, under which conditions the wells will remain stable for 2 months, or until the labeled expiry date, whichever is earlier.
• Seal and return unused calibrators to 2-8 °C, under which conditions the stability will be retained for 1 month, for longer use, store opened calibrators in aliquots and freeze at -20 °C. Avoid multiple freeze-thaw cycles.
• Seal and return all the other unused reagents to 2-8 °C, under which conditions the stability will be retained for 2 months, or until the labeled expiry date, whichever is earlier.
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